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| کلمات کلیدی | AN INVESTIGATION INTO THE RELEASE OF MEDIATORS FROM THE RBL CELLS TRANSFECTED WITH HUMAN CD23 WHEN SENSITISED BY HUMAN IgE N. Nazari1 1Department of Microbiology, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. The CD23 antigen is known to be expressed by a large variety of haematopoietic cells. This antigen has been shown to be implicated in the initiation and the development of parasitic diseases. Mast cells and basophils are important effector cells in T helper 2 cell-dependent, immunoglobulin-E-associated allergic disorders and immune responses to parasites. β-hexosaminidase is a mediator, a proteoglycan synthesised by the cell and contained in the granules from which it is released into the extracellular environment. Cellular activation through human CD23 with IgE was analysed by investigating mediator secretion from the RBL.2H3 cell line expressing human CD23a and b fragments. The Rat basophilic leukaemia, RBL-2H3.1 cell line, an established model for mucosal mast cell tissue and valuable tool for studying IgE-mediated secretion of immunological mediators. Functional analysis of CD23 receptors expressed on RBL-2H3 cells has been reported to demonstrate an increase in serotonin release following crosslinking with antibodies to CD23. Cloning and expression of CD23a and b in RBL-2H3 were successful in the current study and the binding of wild type IgE to transfected cells was observed. The availability of a secretion assay utilising a rat basophilic leukaemia cell line (RBL cells) transfected with the human CD23a and b enabled the assessment of the effect of IgE molecules on cell activation through the low affinity receptor (CD23). In this investigation the results of the cellular secretion assays demonstrated that there was no mediator release (β-hexoseaminidase) was observed through CD23a and b on the transfected cell line RBL-CD23a and RBL-CD23b cells when sensitised by human IgE and cross-linked using rat anti-human IgE antibody to the receptor. |
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