چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | علوم پزشکی کرمانشاه |
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| کتایون درخشنده | اول |
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| کلمات کلیدی | Role of Nanoparticle Carrier in Cellular Uptake, Absorption and Transport of 9-Nitrocamptothecin a New and Potent Anticancer Drug K.Derakhshandeh1,2 , S.Dadashzadeh3, G. Hochhaus1 Department of Pharmaceutics, School of Pharmacy, University of Floride, Gainesville, USA Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, IRAN kderakhshandeh@kums.ac.ir This study evaluated whether the encapsulation into polymeric nanoparticle might improve the oral absorption of the new anticancer drug, 9-nitrocamptohecin (9-NC) by circumventing P-glycoprotein exposure, protect labile molecules from gastrointestinal enzymes and acidic condition and for providing prolonged drug exposure of target cells like cancerous cell. Differnet sizes (110 to 950 nm) of 9-NC nanoparticles using ply (lactic-glycolic acid) (PLGA 50:50) as a biodegradable polymer were prepared by nanoprecipitation method. Transport of nanoparticle across caco-2 cell monolayer as a function of incubation time, temperature and concentration was evaluated for the different nanoparticle formulations. The amount of 9-NC transported from apical to basolateral side and the uptake of drug into the cells was determined by reversed phase HPLC. Uptake of intact nanoparticle into the caco-2 cells was visualized by confocal laser scanning microscopy using 6-coumarin as a fluorescent marker. The study demonstrated that caco-2 cell uptake and transport of encapsulated 9-NC is significantly affected by the diameter of the carrier, incubation time and concentration. The results indicate a significant accumulation of the drug in the cell membrane takes and an enhanced diffusion across the cell membranes and sustained released characterics of polymeric carriers could provide prolonged drug effects. This new formulation especially in lowest size is expected to possess enhanced oral bioavailability from GI tract, that might facilitate oral delivery of 9-nitrocamptothecin. |
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| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
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