چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 6482 |
| عنوان فارسی مقاله | انکپسوله کردن 9- نیتروکمپتوتسین در PLGA-PEG 5% میزان سمیت سلولی آن را در سلول های سرطانی تخمدان انسانی از طریق آپوپتوز افزایش می دهد |
| عنوان لاتین مقاله | Encapsulation in PLGA-PEG 5% enhances 9- Nitrocamptothecin mediated cytotoxicity to human ovarian carcinoma cell line through apoptosis pathway |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | سیزدهمین کنگره بین المللی سم شناسی ایران |
| نوع کنگره / همایش | داخلی |
| کشور محل برگزاری کنگره/ همایش | ایران |
| شهر محل برگزاری کنگره/ همایش | ارومیه |
| سال انتشار/ ارائه شمسی | 1394 |
| سال انتشار/ارائه میلادی | 2015 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1394/02/22 الی 1394/02/24 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | 13irtoxico |
| آدرس علمی (Affiliation) نویسنده متقاضی | 4.0000 |
| نویسنده | نفر چندم مقاله |
|---|---|
| کتایون درخشنده | دوم |
| لیلا حسین زاده | اول |
| اقدس جلالی زاده | |
| فرحناز احمدی | سوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | 9- Nitrocamptothecin; PLGA-PEG nanoparticles; Apoptosis; A2780 ovarian carcinoma cell line |
| چکیده | Ovarian cancer is the fifth leading cause of the cancer-related death among women. 9-nitrocamptothecin (9- NC) is a water-insoluble derivative of camptothecin used for the treatment of patients with advanced ovarian cancer. Previous studies showed that the encapsulation of 9-NC in poly (lactic-co-glycolic acid, PLGA) nanoparticles increased the cytotoxic effect of the drug on different cancer cell lines. In the present study, the cytotoxic effects of 9-NC, 9-NC-loaded PLGA and PLGA-polyethylene glycol (PLGA-PEG) nanoparticles with varying degree of PEG (5, 10, and 15%) were evaluated on human ovarian carcinoma cell line. Furthermore, the mode of cell death induced by 9-NC and the optimized 9-NC-loaded PLGA-PEG nanoparticles on A2780 cell line were investigated. 9-NC incorporating nanoparticles were prepared by nanopercipitation method and their physicochemical characteristics were evaluated using standard methods. The results showed that activation of caspase-3 and -9 significantly increased by free 9-NC and PLGA-PEG loaded nanoparticles in A2780 cells. In contrast to the free drug which increased the activation of caspase-8, 9-NC-loaded PLGA-PEG nanoparticles did not alter the activation of caspase-8. Collectively, it appears that apoptosis induced by 9-NC incorporated in PLGA-PEG 5% occurred through the activation of caspase-9 rather than activation of caspase-8 which is the mediator of extrinsic pathway. Moreover, our results confirmed that 9-NC in nanoparticles at the level of gene expression potentiated down-regulation of Bcl-2, up regulation of Bax, and Smac/DIABLO leading to a decrease in mitochondrial membrane potential. Taken together, our results showed that 9-NC incorporated in PLGA-PEG 5% nanoparticles is able to induce apoptosis in A2780 human ovarian carcinoma cells and has the potential for the treatment of ovarian carcinoma. |
| متن مقاله | |
| نتیجه مقاله | To summarize, 9-NC nanoparticles with 5% PEG-surface modification inhibited the growth of human ovarian carcinoma cells A2780 and induced mitochondria-related apoptosis. The more detailed mechanism of cell apoptosis by 9-NC- loaded PLGA-PEG 5% nanoparticles remains to be elucidated |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| application.pdf | 1394/03/17 | 447273 | دانلود |
| Ticket.pdf | 1394/03/17 | 468006 | دانلود |
| Poster.docx | 1394/03/17 | 175434 | دانلود |
