چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 6684 |
| عنوان فارسی مقاله | Proteomics analysis of human oligodendroglioma proteome |
| عنوان لاتین مقاله | Proteomics analysis of human oligodendroglioma proteome |
| نوع مقاله | مقاله اصیل (پژوهشی، Original) |
| بالاترین نمایه نامه بینالمللی | ISI |
| سطح مقاله | |
| IF | 2.082 |
| عنوان نشریه | Gene |
| نوع نشریه | خارجی ایندکس شده |
| شماره نشریه | 1 |
| دوره | 569 |
| تاریخ انتشار شمسی | 1394/06/01 |
| تاریخ انتشار میلادی | 2015 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.sciencedirect.com/science/article/pii/S0378111915006113 |
| DOI | 10.1016/j.gene.2015.05.039 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran |
| Proteomics analyses enable the identification and quantitation of proteins. Froma purely clinical perspective, the application of proteomics based on innovations, may greatly affect the future management of malignant brain tumors. This optimism is based on four main reasons: diagnosis, prognosis, selection of targeted therapy based on molecular profile of the brain tumor and monitoring therapeutic response, or resistance. We extracted the proteins of tumor and normal brain tissues, and then evaluated the protein purity by Bradford test. In this study, we separated the proteins by two-dimensional (2DG) gel electrophoresis methods. Then spots were analyzed, compared using statistical data and specific software and were identified by pH isoelectric, molecular weights and data banks. The protein profiles were determined using 2D gel electrophoresis and MALDI TOF/TOF mass spectrometry approaches. Simple statistical tests were used to establish a putative hierarchy in which the change in protein level was ranked according to a cut-off point with p b 0.05. The 2D gel showed a total of 1328 spots among which 157 spots were under-expressed and 276 spots were overexpressed. Most proteins are subjects to post-translational modifications, where amino acid residues may be chemically modified or conjugated by small proteins like ubiquitin. Proteomics is a powerful way to identifying multiple proteins which are altered following a neuropharmacological intervention in a CNS disease. |
| نویسنده | نفر چندم مقاله | نویسنده مسئول |
|---|---|---|
| الهام کاظمی | پنجم | خير |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| proteomics.pdf | 1394/04/16 | 735136 | دانلود |
