چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 7886 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | Isoniazid MIC and KatG Gene Mutations among Mycobacterium tuberculosis Isolates in west of Iran |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | هشتمین کنگره بین المللی آزمایشگاه و بالین و اولین کنگره ملی علوم پایه پزشکی و تولید دانش بنیان |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | ایران |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1394 |
| سال انتشار/ارائه میلادی | 2016 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1394/11/17 الی 1394/11/19 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://isaclcongress.ir/Default.aspx |
| آدرس علمی (Affiliation) نویسنده متقاضی | 2.0000 |
| نویسنده | نفر چندم مقاله |
|---|---|
| پرویز مهاجری | دوم |
| حدیث صدری | اول |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Isoniazid Resistance, Kat G, Mycobacterium tuberculosis,MIC |
| چکیده | Background: Isoniazid (INH) is one of the main first line drugs used in treatment of tuberculosis and development of resistance against this compound can result in serious problems in treatment procedures. A major role in the development of resistance of Mycobacterium tuberculosis to isoniazid (INH) is attributed to mutations in the katG gene coding for the catalase/peroxidase, an enzyme required for obtaining a pharmacologically active form of the drug. Analysis of mutations in the katG gene in M. tuberculosis strains may contribute to the development of reliable and rapid tests for detection of INH resistance. Material method: 125 M. tuberculosis isolates were subjected to study of which, 34 strains were INH-resistant and 91 strains were INH-sensitive. DNA extraction, katG amplification, and microarray were performed. Result: Of 25 INH-resistant strains, the mutation was identified in 32% and 14 % in the KatG315 (Ser→Thr) and KatG315 (Ser→Asn) respectively.INH. Minimal inhibitory concentrations (MIC) was <0.2 μg/ml in all sensitive strains whereas among34 INH -resistant isolates, INH MIC was higer than 0.2 μg/ml ranged from 0.2 to 1.2 μg/ml. Conclusion: High levels of INH MIC were observed in the strains which had mutation in the KatG gene in position 315 Our findings revealed that, microarry is able to detect most of INH resistance isolates. It requires simple equipment, and the results are easy to interpret and could be used for rapid identification of INH resistance. |
| متن مقاله | Isoniazid MIC and KatG Gene Mutations among Mycobacterium tuberculosis Isolates in west of Iran Hadis sadri1, Parviz Mohajeri2 1- Kermanshah University of Medical Sciences, Kermanshah, Iran 2- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran * Corresponding author: Hadis Sadri Kermanshah University of Medical Sciences, Kermanshah, Iran. E-mails: hadissadr@yahoo.com Abstract Background: Isoniazid (INH) is one of the main first line drugs used in treatment of tuberculosis and development of resistance against this compound can result in serious problems in treatment procedures. A major role in the development of resistance of Mycobacterium tuberculosis to isoniazid (INH) is attributed to mutations in the katG gene coding for the catalase/peroxidase, an enzyme required for obtaining a pharmacologically active form of the drug. Analysis of mutations in the katG gene in M. tuberculosis strains may contribute to the development of reliable and rapid tests for detection of INH resistance. Material method: 125 M. tuberculosis isolates were subjected to study of which, 34 strains were INH-resistant and 91 strains were INH-sensitive. DNA extraction, katG amplification, and microarray were performed. Result: Of 25 INH-resistant strains, the mutation was identified in 32% and 14 % in the KatG315 (Ser→Thr) and KatG315 (Ser→Asn) respectively.INH. Minimal inhibitory concentrations (MIC) was <0.2 μg/ml in all sensitive strains whereas among34 INH -resistant isolates, INH MIC was higer than 0.2 μg/ml ranged from 0.2 to 1.2 μg/ml. Conclusion: High levels of INH MIC were observed in the strains which had mutation in the KatG gene in position 315 Our findings revealed that, microarry is able to detect most of INH resistance isolates. It requires simple equipment, and the results are easy to interpret and could be used for rapid identification of INH resistance. Keywords: Isoniazid Resistance, Kat G, Mycobacterium tuberculosis,MIC |
| نتیجه مقاله | Of 25 INH-resistant strains, the mutation was identified in 32% and 14 % in the KatG315 (Ser→Thr) and KatG315 (Ser→Asn) respectively.INH. Minimal inhibitory concentrations (MIC) was <0.2 μg/ml in all sensitive strains whereas among34 INH -resistant isolates, INH MIC was higer than 0.2 μg/ml ranged from 0.2 to 1.2 μg/ml. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Lab & Clinic - Oral.pdf | 1394/11/25 | 1087883 | دانلود |
| request 001.jpg | 1394/11/25 | 482399 | دانلود |
| pic 001.jpg | 1394/11/25 | 458717 | دانلود |
