چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 7889 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | Frequency of Point mutations in embB gene associated with resistance to ethambutol in Mycobacterium tuberculosis strains isolated from TB patients in West of Iran (2014-15) |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | هشتمین کنگره بین المللی آزمایشگاه و بالین و اولین کنگره ملی علوم پایه پزشکی و تولید دانش بنیان |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | ایران |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1394 |
| سال انتشار/ارائه میلادی | 2016 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1394/11/17 الی 1394/11/19 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://isaclcongress.ir/Default.aspx |
| آدرس علمی (Affiliation) نویسنده متقاضی | 1.0000 |
| نویسنده | نفر چندم مقاله |
|---|---|
| پرویز مهاجری | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Antibiotic-Resistant, Mycobacterium tuberculosis, Ethambutol, EmbB gene |
| چکیده | Background and Aim : Mutations in embB gene, especially those in the region determines the resistance to ethambutol (ERDR) are considered as 'hot-spots'. These mutations are frequently has been reported in EMB-resistant M. tuberculosis isolates and shown with Sequence analysis. Aim of this study was frequency of Point mutations in embB gene associated with resistance to ethambutol in Mycobacterium tuberculosis strains isolated from TB patients in West of Iran (2014-15) Methods : : This study (a cross-sectional survey) were done on smear positive patients in west region TB research center of Kermanshah city during 2014-15. Out of the 1069 strains of Mycobacterium tuberculosis, 50 strains were selected and evaluated (22 men and 28 women; age range: 23 to 86 years; median age: 54.5 years) with pulmonary TB in this study. Results : No mutations were found in embB gene in the susceptible strains. Mutations in embB gene were found in all of the seven EMB resistance isolates, 5 (42/71%) cases of them were MDR. The most frequent substantiation of amino acid was occurred at codon 306 in 6 (64/85%) of the EMB-resistant isolates. The Met306Val substitution resulting from an A→G transition was detected in 3 (42.85) EMB-resistant isolates, as well as the Met306Ile substitution, due to a G→A transition was detected in 3 (42.85) resistant isolates. Conclusion : The results of this study proximally confirm all of the findings regularly reported in earlier studies. Mutations in embB306 and 406 codons just found in EMB-resistant isolates. substantiation amino acid at codon 306 was most frequent. We not found any mutations in codon 497 and other codons that previous studies have been shown. |
| متن مقاله | PB-181 Frequency of Point mutations in embB gene associated with resistance to ethambutol in Mycobacterium tuberculosis strains isolated from TB patients in West of Iran (2014-15) Rashid Ramazanzadeh1, Parviz mohajeri2, Bahman Mohammadi3 1. Cellular & Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran 2. Department of Microbiology, Kermanshah University of Medical Sciences, kermanshah, Iran 3. Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran. Corresponding Author: Bahman Mohammadi Email: bahman.mo67@gmail.com Background and Aim : Mutations in embB gene, especially those in the region determines the resistance to ethambutol (ERDR) are considered as 'hot-spots'. These mutations are frequently has been reported in EMB-resistant M. tuberculosis isolates and shown with Sequence analysis. Aim of this study was frequency of Point mutations in embB gene associated with resistance to ethambutol in Mycobacterium tuberculosis strains isolated from TB patients in West of Iran (2014-15) Methods : : This study (a cross-sectional survey) were done on smear positive patients in west region TB research center of Kermanshah city during 2014-15. Out of the 1069 strains of Mycobacterium tuberculosis, 50 strains were selected and evaluated (22 men and 28 women; age range: 23 to 86 years; median age: 54.5 years) with pulmonary TB in this study. Results : No mutations were found in embB gene in the susceptible strains. Mutations in embB gene were found in all of the seven EMB resistance isolates, 5 (42/71%) cases of them were MDR. The most frequent substantiation of amino acid was occurred at codon 306 in 6 (64/85%) of the EMB-resistant isolates. The Met306Val substitution resulting from an A→G transition was detected in 3 (42.85) EMB-resistant isolates, as well as the Met306Ile substitution, due to a G→A transition was detected in 3 (42.85) resistant isolates. Conclusion : The results of this study proximally confirm all of the findings regularly reported in earlier studies. Mutations in embB306 and 406 codons just found in EMB-resistant isolates. substantiation amino acid at codon |
| نتیجه مقاله | No mutations were found in embB gene in the susceptible strains. Mutations in embB gene were found in all of the seven EMB resistance isolates, 5 (42/71%) cases of them were MDR. The most frequent substantiation of amino acid was occurred at codon 306 in 6 (64/85%) of the EMB-resistant isolates. The Met306Val substitution resulting from an A→G transition was detected in 3 (42.85) EMB-resistant isolates, as well as the Met306Ile substitution, due to a G→A transition was detected in 3 (42.85) resistant isolates. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Bacteriology-poster.pdf | 1394/11/25 | 1775323 | دانلود |
| request 001.jpg | 1394/11/25 | 482399 | دانلود |
| Congress 001.jpg | 1394/11/25 | 1161662 | دانلود |
