چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | علوم پزشکی کرمانشاه |
| نویسنده | نفر چندم مقاله |
|---|---|
| خیراله یاری | چهارم |
| محمد طاهر مرادی | سوم |
| پریسا ظفری | دوم |
| نسرین ایرانشاهی | اول |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Genetic polymorphism, Gastric cancer, Inflammation, Iran, Interleukin-1β |
| چکیده | Background: Cytokines as the potent mediator in immune system play a critical role in biological activities such as inflammation processes. Inflammation triggering factor in many diseases. one of the pro inflammatory cytokines is IL-1. Recent studies confirmed that interleukin-1β (IL-1β) is highly genetic polymorphic, and a few of the genetic polymorphisms of this cytokine are associated with an increased risk of pathogenesis in gastric cancer. The current study was conducted to assess the influence of IL-1β+3954 genotypes on the risk of gastric cancer in the Iranian population. Material and method: Genomic DNA was isolated from endoscopic fresh biopsy samples in 49 gastric cancer patients and peripheral blood of 53 healthy volunteers. The polymorphism of IL-1β C/T was studied with a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: In the both patients and control group, the genotypes frequencies of IL-1β+3954 gene were in Hardy–Weinberg equilibrium. Based on obtained results, the frequencies of IL-1β+3954 A1A1, A1A2 and A2A2 genotypes in healthy subjects were 26.4, 66 and 7.6 %, respectively. Also, in gastric cancer patients, A1A1, A1A2 and A2A2 with 4.1, 51 and 44.9%, respectively, were observed (p<0.05). Conclusion: Our results showed a significant association between IL-1B+3954 genotypes with the risk of gastric cancer disease in Iranian population. However, further studies with a larger sample size are needed to confirm these results. |
| متن مقاله | Background: Cytokines as the potent mediator in immune system play a critical role in biological activities such as inflammation processes. Inflammation triggering factor in many diseases. one of the pro inflammatory cytokines is IL-1. Recent studies confirmed that interleukin-1β (IL-1β) is highly genetic polymorphic, and a few of the genetic polymorphisms of this cytokine are associated with an increased risk of pathogenesis in gastric cancer. The current study was conducted to assess the influence of IL-1β+3954 genotypes on the risk of gastric cancer in the Iranian population. Material and method: Genomic DNA was isolated from endoscopic fresh biopsy samples in 49 gastric cancer patients and peripheral blood of 53 healthy volunteers. The polymorphism of IL-1β C/T was studied with a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: In the both patients and control group, the genotypes frequencies of IL-1β+3954 gene were in Hardy–Weinberg equilibrium. Based on obtained results, the frequencies of IL-1β+3954 A1A1, A1A2 and A2A2 genotypes in healthy subjects were 26.4, 66 and 7.6 %, respectively. Also, in gastric cancer patients, A1A1, A1A2 and A2A2 with 4.1, 51 and 44.9%, respectively, were observed (p<0.05). Conclusion: Our results showed a significant association between IL-1B+3954 genotypes with the risk of gastric cancer disease in Iranian population. However, further studies with a larger sample size are needed to confirm these results. |
| نتیجه مقاله | Our results showed a significant association between IL-1B+3954 genotypes with the risk of gastric cancer disease in Iranian population. However, further studies with a larger sample size are needed to confirm these results. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| darkhast.docx | 1395/05/26 | 12059 | دانلود |
| U2.png | 1395/05/26 | 351293 | دانلود |
| Untitled.png | 1395/05/26 | 147807 | دانلود |
