چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 10208 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | Identification and differentiation of Campylobacter species by high-resolution melting curve analysis |
| نوع مقاله | مقاله اصیل (پژوهشی، Original) |
| بالاترین نمایه نامه بینالمللی | ISI |
| سطح مقاله | |
| IF | 1.888 |
| عنوان نشریه | Microbial Pathogenesis |
| نوع نشریه | خارجی ایندکس شده |
| شماره نشریه | 7 |
| دوره | 108 |
| تاریخ انتشار شمسی | 1396/04/12 |
| تاریخ انتشار میلادی | |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://dx.doi.org/10.1016/j.micpath.2017.05.009 |
| DOI | 10.1016/j.micpath.2017.05.009 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Department of Microbiology, Kermanshah University of Medical Sciences, Kermanshah, Iran |
| Background: Campylobacter jejuni and Campylobacter coli are the important food-born zoonotic pathogen, also are leading causes of human food borne illnesses worldwide. cadF gene is expressed in all C. jejuni and C. coli strains and mediates cell binding to the cell matrix protein, Fibronectin. Highresolution melting (HRM) analysis is emerging as an efficient and robust method for discriminating DNA sequence variants. The goal of this study was to apply HRM analysis to identification of C. jejuni and C. coli. Materials and methods: A total of 100 samples were obtained from chicken in Kermanshah, Iran. HRM analysis based on cadF gene and Eva Green was developed to the identification of Campylobacter to the species level. Results: Fifty-five of 100 samples were positive as campylobacter (7 C. jejuni, 29 C. coli, 16 mixes and 3 unknown). Minor variations were observed in melting point temperatures of C. coli or C. jejuni isolates and this variation can be used to the differentiation between C. coli or C. jejuni isolates. Conclusions: The results of this study indicated that HRM curve analysis can be a suitable technique and rapid technique for distinguishing between C. jejuni and C. coli isolates |
| نویسنده | نفر چندم مقاله | نویسنده مسئول |
|---|---|---|
| بیژن نعمان پور | سوم | بلي |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| campylobacter NB.pdf | 1396/02/26 | 558212 | دانلود |
