چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 10520 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | High-performance liquid chromatographic determination of lamivudine in human serum using liquid–liquid extraction; application to pharmacokinetic studies |
| نوع مقاله | مقاله اصیل (پژوهشی، Original) |
| بالاترین نمایه نامه بینالمللی | ISI |
| سطح مقاله | هیچکدام |
| IF | 2.603 |
| عنوان نشریه | Journal of Chromatography B, |
| نوع نشریه | خارجی ایندکس شده |
| شماره نشریه | 2 |
| دوره | 832 |
| تاریخ انتشار شمسی | 1384/06/01 |
| تاریخ انتشار میلادی | |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.sciencedirect.com/science/article/pii/S1570023205004435 |
| DOI | |
| آدرس علمی (Affiliation) نویسنده متقاضی | School of pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran |
| A simple, fast, and sensitive high performance liquid chromatographic (HPLC) assay was developed for quantitation of lamivudine in human serum. Lamivudine is polar compound and its extraction from the human serum in previously published HPLC methods involved either protein precipitation or solid phase extraction techniques. However, existence of endogenous peaks which interfere with the drug or appeared as late eluting peaks and lead to long run time of analysis has been reported. Application of either an ion pairing agent in the mobile phase or time consuming column purge has been used in the published methods. Present paper describes liquid – liquid extraction of lamivudine and internal standard (famotidine) using dichloromethane-isopropyl alcohol (1:1, v/v) as an extracting solvent and salting out approach. The mobile phase was a mixture of phosphate buffer (0.05 M) containing triethylamine (1 mL/L, v/v; pH 3.5) and methanol (91:9, v/v) at a flow rate of 2.2 mL/min. The analysis was performed on a column (150mm×6mm i.d.) which was packed with 5m particles of ODS packing material. Under these conditions no interference in the assay from any endogenous substance was observed. The limit of quantification was evaluated to be 5 ng/mL. Accuracy and precision of the method were also studied and the technique was shown to be selective and linear into the concentration range of 5–2500 ng/mL. This method has been used in two randomized crossover bioequivalence studies of 100 and 150 mg lamivudine preparations in 12 and 24 healthy volunteers, respectively. |
| نویسنده | نفر چندم مقاله | نویسنده مسئول |
|---|---|---|
| غلامرضا بهرامی | اول | بلي |
| امیر کیانی | سوم | خير |
| بهاره محمدی | چهارم | خير |
| شهلا میرزایی | دوم | خير |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|
