چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 10553 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | High performance liquid chromatographic determination of topiramate in human serum using UV detection |
| نوع مقاله | گزارش کوتاه (Policy Brife,Short Communication, Short Report, Brief report) |
| بالاترین نمایه نامه بینالمللی | ISI |
| سطح مقاله | از مجلات برتر10%(scopus-Q1) |
| IF | 2.391 |
| عنوان نشریه | Journal of Chromatography B |
| نوع نشریه | خارجی ایندکس شده |
| شماره نشریه | 1 |
| دوره | 822 |
| تاریخ انتشار شمسی | 1384/05/10 |
| تاریخ انتشار میلادی | |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.sciencedirect.com/science/article/pii/S1570023205003971 |
| DOI | |
| آدرس علمی (Affiliation) نویسنده متقاضی | School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran |
| Topiramate has no ultraviolet, visible or fluorescence absorption. Analysis of the drug in human serum has been reported by high performance liquid chromatography (HPLC) with either mass detector or fluorescence detection after precolumn derivatization using 9-fluorenylmethyl chloroformate as fluorescent labeling agent. This study was aimed to validate derivatization and analysis of topiramate in human serum with HPLC using UV detection. The drug was extracted from human serum by liquid–liquid extraction and subjected to derivatization with 9-fluorenylmethyl chloroformate. Analysis was performed on a phenyl column using of spectrophotometer detection operated at wavelength of 264 nm. A mixture of phosphate buffer (0.05 M) containing triethylamine (1 ml/l, v/v; pH 2.3) and methanol (28:72, v/v) at a flow rate of 2.5 ml/min was used as mobile phase. No interference was found with endogenous substances. Validity of the method was studied and the method was precise and accurate with a linearity range from 40 ng/ml to 40 g/ml. The limit of quantification was 40 ng/ml of serum. The correlation coefficient between HPLC methods using fluorescence and UV detections was studied and found to be 0.992 © 2005 Elsevier B.V. All rights reserved. |
| نویسنده | نفر چندم مقاله | نویسنده مسئول |
|---|---|---|
| غلامرضا بهرامی | اول | بلي |
| امیر کیانی | چهارم | خير |
| بهاره محمدی | سوم | خير |
| شهلا میرزایی | دوم | خير |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| High performance liquid chromatographic determination of topiramate.pdf | 1396/04/24 | 84621 | دانلود |
