چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 10554 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | Determination of acyclovir in human serum by high-performance liquid chromatography using liquid-liquid extraction and its application in pharmacokinetic studies. |
| نوع مقاله | مقاله اصیل (پژوهشی، Original) |
| بالاترین نمایه نامه بینالمللی | ISI |
| سطح مقاله | هیچکدام |
| IF | 2.603 |
| عنوان نشریه | Journal of Chromatography B |
| نوع نشریه | خارجی ایندکس شده |
| شماره نشریه | 1 |
| دوره | 816 |
| تاریخ انتشار شمسی | 1385/11/18 |
| تاریخ انتشار میلادی | |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.sciencedirect.com/science/article/pii/S1570023204009572 |
| DOI | |
| آدرس علمی (Affiliation) نویسنده متقاضی | Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran |
| A fast, simple and sensitive high performance liquid chromatographic (HPLC) method has been described for determination of acyclovir in human serum. Since acyclovir is a polar compound and soluble in aqueous medium and practically insoluble in most of organic solvents, its analysis in biological fluids in currently published HPLC methods, involve pre-treatment of acyclovir plasma sample including deproteinization or solid phase extraction. In present method liquid–liquid extraction of acyclovir and internal standard (vanillin) is achieved using dichloromethane-isopropyl alcohol (1:1, v/v) as an extracting solvent. Analysis was carried out on ODS column using methanol-phosphate buffer (0.05 M) containing sodium dodecyl sulfate (200 mg/L) and triethylamine (2 mL/L, v/v) as mobile phase (pH = 2.3; 5:95, v/v) at flow rate of 2 ml/min. The method was shown to be selective and linear into the concentration range of 10–2560 ng/mL. Accuracy and precision of the method were also studied. The limit of quantitation was evaluated to be 10 ng/mL. This method was applied in bioequivalence study of two different acyclovir preparations after administration of 400 mg in 12 healthy volunteers. © 2004 Elsevier B.V. All rights reserved. |
| نویسنده | نفر چندم مقاله | نویسنده مسئول |
|---|---|---|
| غلامرضا بهرامی | اول | بلي |
| امیر کیانی | سوم | خير |
| شهلا میرزایی | دوم | خير |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Determination of acyclovir in human serum by high-performance.pdf | 1396/04/24 | 102297 | دانلود |
