چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 10555 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | Sensitive analytical method for Topiramate in human serum by HPLC with pre-column fluorescent derivatization and its application in human pharmacokinetic studies. |
| نوع مقاله | مقاله اصیل (پژوهشی، Original) |
| بالاترین نمایه نامه بینالمللی | ISI |
| سطح مقاله | از مجلات برتر10%(scopus-Q1) |
| IF | 2.176 |
| عنوان نشریه | Journal of Chromatography B |
| نوع نشریه | خارجی ایندکس شده |
| شماره نشریه | |
| دوره | 813 |
| تاریخ انتشار شمسی | 1383/09/11 |
| تاریخ انتشار میلادی | |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://www.sciencedirect.com/science/article/pii/S1570023204007664 |
| DOI | |
| آدرس علمی (Affiliation) نویسنده متقاضی | Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran |
| A sensitive and specific high performance liquid chromatographic method for quantitation of topiramate in human serum was developed using HPLC with fluorescence labeling reagent. Topiramate was extracted from human serum by dichloromethane and derivatized by reaction with 9-fluorenylmethyl chloroformate (FMOC-Cl) in the presence of borate buffer. Analysis was performed on a CN column with sodium phosphate buffer (pH 2.2) containing 1 ml/l triethylamine and methanol (52:48 (v/v)) as mobile phase. Amantadine was used as internal standard. The standard curve was linear over the range 20–5000 ng/ml of topiramate in human serum. The mean intra-day precision was from 10.5% (low concentration) to 1.2% (high concentration) and the within-day precision from 1.5 to 12.5% determined on spiked samples. The accuracy of the method was 96.5–107.5% (intra-day) and 98.4–105% (inter-day). The limit of quantification was 20 ng/ml of serum. This method was used in a bioequivalence study after administration of 2×25 mg topiramate in 24 healthy volunteers. © 2004 Elsevier B.V. All rights reserved. |
| نویسنده | نفر چندم مقاله | نویسنده مسئول |
|---|---|---|
| غلامرضا بهرامی | اول | بلي |
| امیر کیانی | سوم | خير |
| شهلا میرزایی | دوم | خير |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|
