چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 12624 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | Soluble expression of humanized anti-CD20 single chain antibody in Escherichia coli by cytoplasmic chaperones co-expression |
| نوع مقاله | مقاله اصیل (پژوهشی، Original) |
| بالاترین نمایه نامه بینالمللی | pubmed |
| سطح مقاله | هیچکدام |
| IF | |
| عنوان نشریه | Avicenna Journal of Medical Biotechnology |
| نوع نشریه | داخلی ایندکس شده |
| شماره نشریه | 3 |
| دوره | 10 |
| تاریخ انتشار شمسی | 1397/06/10 |
| تاریخ انتشار میلادی | |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://www.scopus.com/record/display.uri?eid=2-s2.0-85052216519&origin=resultslist&sort=plf-f&src=s&sid=d9301f4b632fc3dc7f5a6f1 |
| DOI | |
| آدرس علمی (Affiliation) نویسنده متقاضی | Department of Medical Biotechnology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran |
| Background: CD20 is an important cell surface receptor that is used for target therapy of B cell lymphoma and some related blood diseases due to vital function of CD20. In previous studies, a Rituximab based humanized single chain variable fragment (scFv) antibody showed good reactivity against B cell related cancer cells. But this recombinant protein produced Inclusion Bodies (IBs) in Escherichia coli (E. coli ) cytoplasm. The aim of this study was to investigate the effect of coexpression with cytoplasmic chaperones on expression and solubility of humanized anti-CD20 scFv in E. coli . Methods: For this purpose, the fragment coding for anti-CD20 huscFv subcloned into the pET22b (+) and transformed into the E. coli BL21 (DE3) was evaluated. In order to inhibit the production of IBs, the effects of co-expression with cytoplasmic chaperones GroEL, DnaK, GroES, Tig, DnaJ and GrpE were investigated. Result: Coexpression with cytoplasmic chaperones led to increased soluble expression of anti-CD20 recombinant protein. Among investigated chaperones, pKJE7 chaperone plasmid containing DnaJ, GrpE, DnaK chaperone genes had significant effects with an expression yield of 325 μ g/ml soluble anti-CD20 scFv. Conclusion: The result of this study demonstrated remarkable effect of pKJE7 chaperone on enhancement of soluble expression of anti-CD20 huscFv antibody in E. coli . |
| نویسنده | نفر چندم مقاله | نویسنده مسئول |
|---|---|---|
| بهمن اکبری | چهارم | خير |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| avesina article.pdf | 1397/07/02 | 495397 | دانلود |
