چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | علوم پزشکی کرمانشاه |
| کد مقاله | 14884 |
| عنوان فارسی مقاله | |
| عنوان لاتین مقاله | Quantitative detection of SRY‑Box 21 (SOX21) gene promoter methylation as a stool‑based noninvasive biomarker for early diagnosis of colorectal cancer by MethyLight method |
| نوع مقاله | مقاله اصیل (پژوهشی، Original) |
| بالاترین نمایه نامه بینالمللی | ISI |
| سطح مقاله | هیچکدام |
| IF | 0.765 |
| عنوان نشریه | Indian Journal of Cancer |
| نوع نشریه | خارجی ایندکس شده |
| شماره نشریه | Issue 2 |
| دوره | Volume : 58 |
| تاریخ انتشار شمسی | 1400/03/17 |
| تاریخ انتشار میلادی | |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://www.indianjcancer.com/article.asp?issn=0019-509X;year=2021;volume=58;issue=2;spage=217;epage=224;aulast=Moradi |
| DOI | DOI: 10.4103/ijc.IJC_37_19 |
| آدرس علمی (Affiliation) نویسنده متقاضی |
| Abstract INTRODUCTION: In recent years, the study of potential epigenetic biomarkers in feces has been an attractive research approach for the noninvasive diagnosis of colorectal cancer (CRC). AIM: The aim of this study was to evaluate the stool‑based DNA methylation potential of SRY‑Box 21 (SOX21) gene promoter as an appropriate candidate biomarker for differentiating CRC patients and healthy individuals for the first time. METHODS: The MethyLight method was performed to analyze the methylation status of SOX21 gene promoter in fecal samples from 40 patients with CRC and 40 healthy controls. In addition, the diagnostic efficiency of measuring the hypermethylated SOX21 gene in the feces to the fecal occult blood test (FOBT) was compared. RESULTS: The percentage of methylated reference (PMR) values in the stool of CRC patients (median 1.44) was higher than those of healthy individuals (median 0) (P < 0.001). A sensitivity of 72.5% and specificity of 100% were obtained for SOX21 gene promoter methylation status and 29 of the patients were considered as positive in methylation status. There was no significant association between PMR values and demographic/clinicopathological features (P > 0.05). CONCLUSION: In summary, the results of the present study demonstrated that the stool‑based assay of SOX21 gene promoter methylation has relatively high sensitivity and specificity and it may serve as a noninvasive biomarker for early detection of CRC. However, more studies with a wide range of samples are required to further confirm the role of hypermethylation of SOX21 in the early CRC diagnosis. |
| نویسنده | نفر چندم مقاله | نویسنده مسئول |
|---|---|---|
| نایب علی رضوانی | پنجم | بلي |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Article.pdf | 1400/04/09 | 578265 | دانلود |
